在exon 3中插入了新霉素选择座。对来自纯合小鼠脾、心脏和肝脏的样本进行 Western blot 分析后,结果显示这个等位基因没有产生可检测到的蛋白质。(来源:J:58609)
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A neomycin selection cassette was inserted into exon 3. Western blot analysis on samples derived from spleen, heart and liver of homozygous mice demonstrated that no detectable protein was produced from this allele. (J:58609)