一种新霉素抗性基因座替换了包含起始翻译位点的exon 2。使用多克隆抗体的 Western blot 分析并未在纯合突变小鼠的睾丸中检测到蛋白质(来源:J:73658)。
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A neomycin resistance cassette replaced exon 2, which contains the translation initiation site. Western blot analysis using polyclonal antibodies did not detect protein in testes from homozygous mutant mice. (J:73658)