Camk2a^tm2Sva

Point mutations were introduced into the gene that altered codon 286 from one encoding threonine to one encoding alanine. This mutation had been shown in vitro to eliminate autophosphorylation at this site and result in the elimination of calcium-calmodulin independent activity without affecting calcium-calmodulin dependent activity. An adjacent loxP-flanked neomycin cassette was removed by transient Cre mediated recombination in ES cells prior to the production of chimeric mice. Western blot and immunohistochemistry experiments on homozygous mice confirmed that a stable mutant protein was produced from this allele. (J:45750)