包含前两个外显子的基因片段被替换成诺卡因选择标记元件。对来自纯合小鼠脑组织的提取物进行 Western 印迹分析显示,这个等位基因不产生可检测到的蛋白质。(来源:J:70570)
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A genomic fragment containing the first two exons was replaced with a neomycin selection cassette. Western blot analysis on brain extracts derived from homozygous mice demonstrated that no detectable protein is produced from this allele. (J:70570)