Bid^tm1Sjk

A 4.2 kb genomic fragment containing exons 1, 2 and part of 3 was replaced with a neomycin selection cassette inserted in reverse transcriptional orientation to the gene. The deleted sequences encode the BH3 domain of the protein. Western blot analysis on spleen and thymus samples derived from homozygous mice demonstrated that no detectable protein is produced from this allele. (J:57073)