在第一个编码外显子中插入了新霉素筛选 cassette,对来自纯合小鼠的脑组织提取的粗膜进行 Western 印迹分析后,未检测到编码蛋白。(来源:J:21121)
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A neomycin selection cassette was inserted into the first coding exon. Western blot analysis on crude membrane preparations of brain derived from homozygous mice demonstrated that no encoded protein was detectable. (J:21121)