包含14号外显子的基因片段被替换成诺卡因选择标记。对雄性小鼠精液的 Western 印迹确认了纯合子中完整蛋白的缺失。(来源:J:69282)
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A genomic fragment containing exon 14 was replaced with a neomycin selection cassette. Western blots on sperm extracts confirmed the absence of the full length protein in homozygous mice. (J:69282)