在来自各种组织的同源小鼠的提取物中,通过盐水介质筛选,没有检测到完整或部分蛋白质的 Western blot 阳性结果,这个系统替换了一段编码第一个 ATP 结合域的序列。(来源:J:44110)
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A hygromycin selection cassette replaced sequences encoding the first ATP binding domain. No full length or truncated protein was detected in western blots on extracts derived from a variety of tissues from homozygous mice. (J:44110)