将耐氨苄青霉素抗性基因 cassette插入了 laminin 2 链域IV编码区域的外显子中。对纯合小鼠的lysate进行 Western blot分析后,结果显示该蛋白质并未存在。(来源:J:43145)
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A neomycin resistance cassette was inserted into an exon that encodes a part of domain IV of the laminin 2 chain. Western blot analysis of lysates derived from homozygous mice demonstrated that the protein was not present. (J:43145)