Ephb2^tm1Paw

A PGK-neomycin resistance cassette replaced 1.4kb of sequence including the exon that encodes amino acids 29-50, and introduced a frame shift downstream of the insertion. In an immune-complex in vitro tyrosine kinase assay, antiserum raised against the C-terminal of the protein did not detect wild-type protein in lysates of homozygous mutant E10.5 embryos. (J:34200)