一个新霉素抗性 cassette 替换了编码 DNA 结合和 leucine 锁定区域的 703bp 序列。E9.5 期胚胎的 Western 印迹分析在纯合突变胚胎中未检测到野生型蛋白。(来源:J:14538)
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A neomycin resistance cassette replaced 703 bp of sequence that codes for the DNA binding and leucine zipper domains. Western blot analysis of E9.5 lysates did not detect wild-type protein in homozygous mutant embryos. (J:14538)