一种新霉素抗性 cassette 替换了 3.6kb 的序列,包括 exon 2。 Western blot 分析并未在纯合突变小鼠的肝脏提取物中检测到该蛋白。(来源:J:32538)
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A neomycin resistance cassette replaced 3.6 kb of sequence, including exon 2. Western blot analysis did not detect the protein in liver extracts from homozygous mutant mice. (J:32538)