在第二内含子插入了loxP位点,第六内含子插入了loxP标记的诺卡因选择子。在将嵌合小鼠产生前,通过在ES细胞中短暂表达Cre重组酶移除了诺卡因选择子,剩下两个位于3-6号外显子周围的loxP位点。Northern blot结果显示,这个等位基因产生了正常长度的转录本,而western blot分析证实了产生了正常的蛋白质。(来源:J:69463)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
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1
22
79

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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