在该基因的exon 1中插入了一个PGK-neomycin cassette。使用抗Mapt抗体对大脑、小脑和坐骨神经干的粗提物进行 Western blot 检测时,未检测到Mapt蛋白。(来源:19030)
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A PGK-neomycin cassette was inserted into exon 1 of the gene. Mapt protein was not detected by Western blot using anti-Mapt antibodies in crude extracts from cerebrum and cerebellum or the dorsal root ganglia of homozygous mutant mice. (J:19030)