将exon 4替换为PGK-Neo cassette。使用针对N端的多克隆抗体进行 Western 分析,在纯合突变动物中未能检测到该蛋白质。(来源:J:20550)
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Replacement of exon 4 with a PGK-Neo cassette. Western analysis using a polyclonal antibody directed against the N-terminus failed to detect the protein in homozygous mutant animals. (J:20550)