在第一个编码外显子中插入了loxP介导的neo cassette, Western blot结果显示纯合突变胚胎中未检测到蛋白表达。(来源:J:67826)
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A loxP flanked neo cassette was inserted in the first coding exon. Western blot analysis confirmed that no protein was expressed in homozygous mutant embryos. (J:67826)