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中文
在exon 5的编码区域引入了点突变，将145和146位的氨基酸从异亮氨酸和甲硫氨酸替换为缬氨酸和缬氨酸，同时增加了BstEII的限制酶切割位点。在exon 4中插入了含lox-P标记的诺卡因 cassette。F2小鼠在使用特异的exon 5引物扩增基因组DNA，并用相应限制酶切割后，表现出预期的靶向等位基因的遗传多样性。对总脑RNA进行Northern blot分析，使用Psen1特异性抗体检测，结果显示纯合突变鼠中，目标等位基因的表达处于正常生理水平。(来源：J:51950)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
