在E9.5同源嵌入体的提取物中进行 Western blot 分析,结果显示这个lacZ基因在第7外显子后面与一个诺卡因抗性基因座融合,且该等位基因未检测到表达的蛋白质。(来源:J:64178)
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A lacZ gene was fused in frame into exon 7 followed by a neomycin resistance cassette. Western blot analysis on extracts derived from E9.5 homozygous embryos confirmed that no detectable protein is expressed from this allele. (J:64178)