在新霉素抗性 cassette 的插入下,破坏了编码蛋白质催化区域部分的两个外显子。针对目标基因的胚胎纤维细胞 Western �印迹结果显示,无法检测到蛋白质。(来源:J:48772)
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Insertion of a neomycin resistance cassette disrupted two exons encoding part of the catalytic domain of the protein. Western blots of embryonic fibroblasts from homozygous mutant mice showed no detectable protein for the targeted gene. (J:48772)