在转录起始点下游于第1外显子插入了新霉素抗性基因座。对纯合小鼠脑组织的 Western blot 分析显示,这个等位基因未检测到可检测到的蛋白表达。(来源:J:44638)
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A neomycin resistance cassette was inserted into exon 1, downstream of the translation initiation start site. Western blot analysis on brain extracts from homozygous mice confirmed that no detectable protein is expressed from this allele. (J:44638)