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A neomycin resistance gene cassette replaced 1.1kb of sequence containing exon 2, which encodes the first zinc finger of the DNA binding domain. RT-PCR and Western blot analysis of intestinal tissue from homozygous mice detected the presence of a truncated transcript and protein that appears to use M53 in exon 3 as an initiation site. This truncated protein is able to bind ligand but lacks transactivation activity. (J:42054, J:129684)