Vil1^tm1Syr

The open reading frame was disrupted by the insertion of a neomycin selection cassette into exon 2. Normal protein was shown to be absent and no aberrant transcripts were detected via Northern blot and RT-PCR analysis of homozygous mutant mice. Western blot analysis confirmed the absence of encoded protein extracts from intestinal and renal tissue of homozygous mutant mice. (J:57346)