内源性起始密码子及编码同源盒的exon被替换成lacZ和pgk-诺卡因载体。野生型蛋白缺失通过在纯合突变胚胎中进行 Western 分析得到确认。(来源:J:49857)
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The endogenous start codon as well as the exon encoding the paired box was replaced a LacZ and PGK-neomycin based cassette. Lack of wild-type protein was confirmed by Western analysis in homozygous mutant embryos. (J:49857)