在基因的第四外显子中插入了新霉素抗性基因 cassette。通过 Western blot 分析和从脾脏和睾丸细胞分离出的细胞进行的活性 blot 分析,发现纯合小鼠中没有 ADPRT1 蛋白存在。(来源:J:41742)
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A neomycin resistance cassette was inserted into the fourth exon of the gene. No ADPRT1 protein was present in homozygous mice as assayed by Western blot analysis and activity blot analysis using cells isolated from spleen and testis. (J:41742)