Del(2Hoxd11-Hoxd13)29Ddu

This allele involved the use of an embryonic stem cell line carrying an ectopic Hoxd11/lacZ transgene located between the resident Hoxd13 and Evx2 genes and flanked at the 3' end by a unique loxP site. A loxPneoloxP cassette was recombined on the same chromosome, downstream of resident Hoxd11. After treatment with Cre recombinase, the resultant deletion removed Hoxd11, Hoxd12, and Hoxd13, but brought the ectopic Hoxd11/lacZ transgene back to the original Hoxd11 locus. Mice homozygous for this deficiency lacked Hoxd11, Hoxd12, and Hoxd13 loci. (J:36443)