在编码激酶域起始位置的序列中插入了新霉素抗性基因 cassette,对E8.5胚胎的lysate进行 Western blot 分析后发现,纯合子中该蛋白缺失。(来源:J:29273)
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A neomycin resistance cassette was inserted into sequences encoding the beginning of the kinase domain. Western blot analysis of lysates derived from E8.5 embryos demonstrated that the protein was absent in homozygotes. (J:29273)