设计了一个靶向载体,目标是去除包含exon 1部分和intron 1部分的0.4kb片段,带有诺卡因抗性基因。 Western blot在突变体中未能检测到蛋白质。(来源:J:51151)
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A targeting vector was designed to delete a 0.4 kb fragment containing part of exon 1 and a part of intron 1, with a neomycin resistance cassette. Western blot failed to detect protein in mutants. (J:51151)