Enables several functions, including ATP binding activity; alanine-tRNA ligase activity; and tRNA binding activity. Involved in alanyl-tRNA aminoacylation. Located in cytoplasm. Human ortholog(s) of this gene implicated in Charcot-Marie-Tooth disease axonal type 2N and developmental and epileptic encephalopathy 29. Orthologous to human AARS1 (alanyl-tRNA synthetase 1). [provided by Alliance of Genome Resources, Apr 2022]