Enables several functions, including 6-phosphofructo-2-kinase activity; anion binding activity; and fructose-2,6-bisphosphate 2-phosphatase activity. Involved in several processes, including carbohydrate phosphorylation; positive regulation of glucokinase activity; and response to peptide hormone. Part of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex. Biomarker of hyperinsulinism. Orthologous to human PFKFB1 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1). [provided by Alliance of Genome Resources, Apr 2022]