Predicted to enable several functions, including 4 iron, 4 sulfur cluster binding activity; FMN binding activity; and tRNA-4-demethylwyosine synthase activity. Predicted to be involved in wybutosine biosynthetic process. Predicted to act upstream of or within tRNA processing. Is expressed in several structures, including alimentary system; brain; cardiovascular system; genitourinary system; and respiratory system. Orthologous to several human genes including TYW1 (tRNA-yW synthesizing protein 1 homolog). [provided by Alliance of Genome Resources, Apr 2022]