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ENTool Introduction and Background
The Sequence Processor is a versatile bioinformatics tool capable of generating reverse, complementary, or reverse-complementary sequences for DNA or RNA. It can also translate these sequences into corresponding amino acid sequences. This tool facilitates rapid processing of sequences of interest, making it suitable for molecular biology research, sequence alignment, and protein prediction.
Detailed Operating Steps
1、Accessing the Tool Interface
Open a browser and navigate to: https://rddc.tsinghua-gd.org/zh/tool/sequence-processor to access the Sequence Processor homepage.
2、Translation Function Demonstration
Select the "Translation" button on the left side. Paste the target sequence into the input box and click [Submit].
3、Viewing and Analyzing Results
The results will display all possible protein sequences, covering all reading frames for both the 5'-3' forward strand and the 3'-5' reverse strand. Excess nucleotides that do not form a complete codon will be highlighted in yellow. (Note: Excess nucleotides refer to terminal nucleotides that cannot form complete codons due to an open reading frame (ORF) termination or a sequence length that is not a multiple of 3.) Results can be downloaded as a .CSV file or copied directly (both three-letter and single-letter amino acid sequences are supported).
4、Other Function Descriptions
Sequence Complement: Select the "Sequence Complement" function, input a DNA or RNA sequence, and submit to obtain its complementary sequence. For example, the complementary sequence of the DNA sequence "ATGC" is "TACG."
Reverse Complement: Select the "Reverse Complement" function, input a sequence, and the tool will first reverse the sequence, then generate its complementary sequence. For example, the reverse complementary sequence of "ATGC" is "GCAT."
Reverse Sequence: Select the "Reverse Sequence" function, input a sequence, and the tool will provide the reverse order of the original sequence. For example, the reverse sequence of "ATGC" is "CGTA."
Auxiliary Functions and Resources
Video Tutorial: Click the [Video Tutorial] link on the page to watch an official operation demonstration.
Applications
Research: Analyzing unknown gene sequences to predict encoded proteins; aligning viral genomes to track mutation sites.
Education: Simulating the central dogma to demonstrate sequence translation; practicing sequence processing operations to reinforce molecular biology understanding.
