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ENTool Introduction and Background
The Sequence Viewer is a bioinformatics tool developed based on the human reference genome, designed to provide users with efficient and accurate gene sequence query and analysis capabilities. The tool enables users to quickly search for target gene sequences, including mRNA, CDS, and amino acid sequences. Through an intuitive visualization interface, it displays gene structure, sequence characteristics, and associated annotation information, meeting research needs in fields such as molecular biology and genetics.
Detailed Operating Steps
1、Enter the Tool Interface
Open a browser and navigate to: https://rddc.tsinghua-gd.org/zh/tool/sequence to access the Sequence Viewer homepage.
2、Searching for Your Target Gene
Enter the target gene name into the search box and click the magnifying glass icon to navigate to a list of relevant genes (including human, mouse, and rat genes). Locate and click on the target gene to proceed to its gene sequence information page.
3、Sequence Information Page
Once you're on the sequence information page, you'll see three function buttons in the top left corner. Click these to freely switch between viewing the target gene's Pre-mRNA, cDNA, and Protein/Codon sequences. Right next to these buttons, a dropdown box lets you select different transcripts. When you choose one, the sequence information on the page updates in real-time, making it easy to compare differences. Need to save a sequence? The download function on the page allows you to get the full-length sequence with a single click, accommodating various needs. The top right corner of the sequence information page also supports locating mutation sites within the sequence.
4、Locating and Copying Sequences
On the sequence information page, you have two ways to precisely pinpoint target regions: you can either directly enter a specific sequence section (like Exon2) or a mutation site into the location box in the top right, or you can click and select it directly on the visualization map. Once located, the corresponding sequence will automatically highlight, making it easy to quickly identify and analyze. After locating, find the [Copy] button on the page and click it to copy the specified segment to your clipboard, ready for pasting elsewhere.
5、Multi-dimensional Sequence Analysis
Jump to AI Analysis Tool: When viewing a sequence, hovering your mouse over any base or amino acid will bring up an information card. Clicking the link or button on this card will take you directly to the AI analysis tool. Here, you can leverage its powerful functions to deeply analyze sequences and uncover more valuable information. (For details and usage, refer to the user manuals for the two AI analysis tools.)
Switch to Mutation Viewer: On the sequence information page, use the mutation site localization function to quickly pinpoint mutation locations. Click the Mutation Information button in the top left of the page to switch to the Mutation Viewer interface. This allows for multi-dimensional analysis of sequence mutations, including known mutation sites and the impact of mutations on gene function, helping you gain a comprehensive understanding of gene sequence characteristics. (For details and usage, refer to the Mutation Viewer user manual.)
Precautions
Gene Naming Convention: Enter standard gene symbols (e.g., "TP53" not "p 53"). You can confirm the correct name via NCBI Gene.
Mutation Site Format: Follow HGVS naming rules (e.g., "c.123A>T" for a coding region mutation, "g.456C>G" for a genomic mutation). Incorrect formats may lead to parsing failures.
Auxiliary Functions and Resources
Video Tutorial: Click the [Video Tutorial] link on the page to watch the official operation demonstration.
Applications
Research: Analyzing gene transcription and translation mechanisms; studying gene transcriptional regulatory elements; assisting with molecular cloning and gene editing; pinpointing pathogenic sites for genetic diseases.
Clinical: Diagnosing monogenic hereditary diseases; matching targeted therapy regimens; guiding personalized medication; assessing prenatal disease risk.
Education: Explaining molecular biology theories; training bioinformatics skills; demonstrating principles of evolutionary biology; hands-on interpretation of sequence characteristics.
Mouse and Rat Research: Optimizing disease model strategies; identifying experimental rodent genotypes; predicting drug targets; validating gene function mechanisms.
