Wrn is located on chromosome 8 of mice. CRISPR/Cas technology was used to design sgRNA; Wrn knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.
查看原文 参与反馈
Wrn is located on chromosome 8 of mice. CRISPR/Cas technology was used to design sgRNA; Wrn knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.