En1 is located on chromosome 1 of mice. CRISPR/Cas technology was used to design sgRNA; En1 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.
查看原文 参与反馈
En1 is located on chromosome 1 of mice. CRISPR/Cas technology was used to design sgRNA; En1 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.