Snca^{tm1.1(SNCA)Mdk}

The wild -type SNCA-GR targeting vector is designed to insert a 158 kb (Chr4:89707185 - 89865027) fragment of the human SNCA in place of Chr6:60683985 - 60848702 of the mouse Snca gene. The genomic sequences are from human assembly GRCh38.p14 and the mouse assembly GRCm39. The construct includes an loxP-flanked neomycin selection cassette. In addition, there is also a SNCA (non-coding antisense RNA) in the human genome segment. The insertion disrupts Snca transcription. Transient cre-mediated recombination removes the neo cassette. (J:101977)