Rr467^em1Nses

CRISPR/Cas9 technology replaced one of the cis-regulatory elements marked as DNase I-hypersensitive sites around the mouse Cbln2 locus designated as the Cbln2 enhancer E2 (1,005 bp) with the corresponding human CBLN2 enhancer E2 (chr18: 72,530,47372,531,324, GRCh38/hg38). Quantitative RT-PCR showed increased RNA expression of mouse Cbln2 in neonatal neocortex in homozygous mice. (J:331714)