Vipr2^em1(cre)Hze

The donor vector sequences contained, from 5' to 3', a partial Vipr2 sequence spanning intron 12 up to and including the endogenous stop codon in exon 13, an internal ribosome entry site 2 sequence (IRES2; allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette, a frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. In addition, several basepair mutations were introduced into the first 30 nucleotides of the Vipr2 3' UTR within exon 13 (to protect the donor vector from CRISPR/Cas9 cleavage). (J:260362)