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A CAG promoter, loxP-flanked neomycin 6xSV40 pA cassette, and SARS-CoV-2 nucleocapsid (N) gene with an HA tag, along with a CAG promoter driving expression of human ACE2 cDNA in the opposite orientation were inserted into intron 1. A BGH poly(A) sequence was added after the SARS-CoV-2 N gene to enhance mRNA stability. The SARS-CoV-2 N gene is not expressed until the removal of the loxP-flanked neomycin cassette via cre-mediated recombination. Expression of the human ACE2 gene was confirmed by immunofluorescence staining, Western blot, and qPCR. (J:338826)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
