Astl^em1Lwa

Leucine codon 184 (CTC) in exon 6 was changed to histidine (CAC) (c.551T>A, p.L184H) using an sgRNA (targeting GTACGTGCATGAGCTCATGTAGG) and an ssODN template with CRISPR/Cas9 technology. The mutation is the equivalent of the same human mutation associated with female infertility. (J:342780)