The targeting construct replaced the coding region of the gene with a Cre recombinase open reading frame followed by an FRT-flanked neomycin cassette. Flp-mediated recombination removed the neo cassette. (J:343535)
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The targeting construct replaced the coding region of the gene with a Cre recombinase open reading frame followed by an FRT-flanked neomycin cassette. Flp-mediated recombination removed the neo cassette. (J:343535)