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A FRT-flanked PGK-Neo-SV40pA selection cassette inserted a strong transcriptional stop signal (3xpA) downstream of a phylogenetically conserved region of the GATA4 bound first heart field specific enhancer. The selection cassette was removed via flp-mediated recombination. This insertion causes a premature termination of transcription and a severely shortened transcript. RNA-seq and qPCR confirmed the absence of full-length RNA in E9.5 hearts. The expression level of its cis located gene Nkx2-5 is unchanged in E9.5 hearts. And the lncRNAs IRENE-SS and IRENE-div expression levels are not affected. (J:342171)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
