Glutamine codon 1963 (CAG) in exon 26 was changed to a stop codon (TAG) (p.Q1963*) and a sequence coding for a 3x FLAG tag was inserted immediately upstream of codon 1963 using an sgRNA and an ssODN template with CRISPR/Cas9 technology. (J:342674)
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Glutamine codon 1963 (CAG) in exon 26 was changed to a stop codon (TAG) (p.Q1963*) and a sequence coding for a 3x FLAG tag was inserted immediately upstream of codon 1963 using an sgRNA and an ssODN template with CRISPR/Cas9 technology. (J:342674)