Dnm1l^tm1.1Tac

A loxP site was inserted into intron 14 (with reference to ENSMUST00000023477) and human GH gene poly(A) signal sequences and a second loxP site were inserted downstream of exon 18. Genomic sequence containing the 3 end of intron 14 and the entirety of exons 15-18 and intervening introns, where serine codon 600 (TCT) in exon 16 (in ENSMUST00000023477) was changed to alanine (GCT) (p.S600A), was inserted downstream of the second loxP site. Subsequent Cre-mediated recombination removed the wild-type exons and inserted poly(A) signal sequences. The mutation blocks phosphorylation of the affected residue in the encoded peptide. (J:342717)