Exons 2-3 were flanked by loxP sites and a neomycin selection cassette flanked by FRT sites was inserted upstream of the second loxP site. The neomycin cassette was removed via flp-mediated recombination. (J:341806)
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Exons 2-3 were flanked by loxP sites and a neomycin selection cassette flanked by FRT sites was inserted upstream of the second loxP site. The neomycin cassette was removed via flp-mediated recombination. (J:341806)