The targeting construct contains a CAG-reversed [loxP-pA-PGK-Neomycin-STOP-loxP]-FLIM-AKAR-WPRE cassette was introduced to intron 1. The Fluorescence Lifetime Imaging Microscopy compatible A Kinase Activity Reporter (FLIM-AKAR) optical reporter (based on the synthetic reporter found in Addgene plasmid# 63058) consists of a donor fluorophore of monomeric enhanced green fluorescent protein (mEGFP) and an acceptor fluorophore of dark yellow fluorescent protein (sREACH). (J:343075)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
129S6/SvEvTac x C57BL/6J
Targeted
Insertion
--
--
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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