The core promoter and exon 1 were flanked by loxP sites and an FRT-flanked neomycin cassette was inserted upstream of the second loxP site. The neomycin selection cassette was removed via flp-mediated recombination. (J:291125)
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The core promoter and exon 1 were flanked by loxP sites and an FRT-flanked neomycin cassette was inserted upstream of the second loxP site. The neomycin selection cassette was removed via flp-mediated recombination. (J:291125)