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Sequence coding for an HA tag was inserted immediately downstream of the ATG start codon in exon 2, a loxP site flanked neomycin resistance gene cassette was inserted into intron 1 and the following mutations were engineered in exon 2: serine codons 535 (TCG), 347 (TCC), 350 (TCA) and 355 (TCT) and threonine codons 338 (ACA), 341 (ACC), 352 (ACA) and 361 (ACC) were changed to alanine (p.S335A, p.S347A, p.S350A, p.S355A, p.T338A, p.T341A, p.T352A and p.T361A). The neo cassette was removed through subsequent Cre-mediated recombination. The mutations block phosphorylation of the affected residues in the encoded peptide. (J:275611)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
