A loxP site was inserted upstream of exon 1 and an FRT-flanked neomycin cassette and second loxP site were inserted downstream of exon 1. The neomycin cassette was removed via flp-mediated recombination leaving exon 1 floxed. (J:328938)
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A loxP site was inserted upstream of exon 1 and an FRT-flanked neomycin cassette and second loxP site were inserted downstream of exon 1. The neomycin cassette was removed via flp-mediated recombination leaving exon 1 floxed. (J:328938)