Gt(ROSA)26Sor^{tm2(CAG-Egfr*L860R)Rnem}

The targeting cassette includes (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette including neomycin sequence, Egfr mutant cDNA (L860R mutation), mCerulean3 (mC3) sequence, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), and polyA signal. This targeting vector was inserted into intron 1 of the Gt(ROSA)26Sor locus. The murine L860R mutation corresponds to the human L858R mutation. (J:333112)