Htr2a^em3(cre)Blr

A cDNA encoding EGFP (flanked on both ends by a serine- and glycine- rich linker) was inserted into the C-terminus (CT) of the mouse Htr2a gene after residue 452 (encoded by exon 3; chr14:74706337 in the GRCm38/mm10 genome assembly) and an IRES-Cre cassette was placed between the stop codon and the 3' UTR (chr14:74706397). A single humanizing A242S (nucleotide: GCA to TCC) mutation was also introduced to exon 3 of the mouse Htr2a gene. The allele was created using CRISPR/cas9 methodologies in C57BL/6J embryos. (J:341620)